Deformed grids for single-particle cryo-electron microscopy of specimens exhibiting a preferred orientation
نویسندگان
چکیده
منابع مشابه
A Primer to Single-Particle Cryo-Electron Microscopy
Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need for crystals. Recent advances in detector technology and software algorithms now allow images of unprecedented quality to be recorded and structures to be determined at near-atomic resolution. However, compared with X-ray crystallography, c...
متن کاملCryo-electron microscopy of vitrified specimens.
Present addresses: ' Centre de Microscopie Electronique (CME), 27 Rue du Bugnon, CH-105 Lausanne; Laboratoire Europeen de Biologie Moleculaire, I.L.L. 156X; F-38042 Grenoble Cedex; ' The Institute of Biophysics, Academica Sinica, Peking, China; 4 Institut de Biochimie, n Rue Humann, F-67000 Strasbourg; 5 Centre de Genetique Moleculaire, C.N.R.S., F-9H90 Gif/Yvette; 6 Howard Hughes Medical Insti...
متن کاملSingle-particle imaging of macromolecules by cryo-electron microscopy.
Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (i.e., unordered, nonaggregated) form is a new approach to the study of molecular assemblies, which are often too large and flexible to be amenable to X-ray crystallography. New insights into biological function on the molecular level are expected from cryo-EM applied to the study of such complexes "trapped" at differ...
متن کاملSingle-particle cryo-electron microscopy of Rift Valley fever virus.
Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV...
متن کاملSingle-particle electron cryo-microscopy: towards atomic resolution.
4. Single particles and angular reconstitution 323 4.1 Preliminary filtering and centring of data 323 4.2 Alignments using correlation functions 324 4.3 Choice of first reference images 324 4.4 Multi-reference alignment of data 325 4.5 MSA eigenvector/eigenvalue data compression 328 4.6 MSA classification 330 4.7 Euler angle determination (‘ angular reconstitution ’) 332 4.8 Sinograms and sinog...
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ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2013
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2013.03.005